Monolithic dual-wedge prism-based spectroscopic single-molecule localization microscopy.

ABSTRACT: By manipulating the spectral dispersion of detected photons, spectroscopic single-molecule localization microscopy (sSMLM) permits concurrent high-throughput single-molecular spectroscopic analysis and imaging. Despite its promising potential, using discrete optical components and managing the delicate balance between spectral dispersion and spatial localization compromise its performance, including non-uniform spectral dispersion, high transmission loss of grating, high optical alignment demands, and reduced precision. We designed a dual-wedge prism (DWP)-based monolithic imaging spectrometer to overcome these challenges. We optimized the DWP for spectrally dispersing focused beam without deviation and with minimal wavefront error. We integrated all components into a compact assembly, min

SUBMITTER: Song KH 

PROVIDER: S-EPMC9307059 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature