Unknown

Dataset Information

0

Antibacterial Peptide NP-6 Affects Staphylococcus aureus by Multiple Modes of Action.


ABSTRACT: Our previous study extracted and identified an antibacterial peptide that was named NP-6. Herein, we investigated the physicochemical properties of NP-6, and elucidated the mechanisms underlying its antimicrobial activity against Staphylococcus aureus. The results showed that the hemolysis activity of NP-6 was 2.39 ± 0.13%, lower than Nisin A (3.91 ± 0.43%) at the same concentration (512 µg/mL). Negligible cytotoxicity towards RAW264.7 cells was found when the concentration of NP-6 was lower than 512 µg/mL. In addition, it could keep most of its activity in fetal bovine serum. Moreover, transmission electron microscopy, confocal laser scanning microscopy, and flow cytometry results showed that NP-6 can destroy the integrity of the bacterial cell membrane and increase the membrane permeability. Meanwhile, NP-6 had binding activity with bacterial DNA and RNA in vitro and strongly inhibited the intracellular β-galactosidase activity of S. aureus. Our findings suggest that NP-6 could be a promising candidate against S. aureus.

SUBMITTER: Hou X 

PROVIDER: S-EPMC9319634 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7913509 | biostudies-literature
2018-04-19 | GSE113302 | GEO
| S-EPMC6008372 | biostudies-literature
| S-EPMC6736180 | biostudies-literature
| S-EPMC2346702 | biostudies-literature
| S-EPMC6274078 | biostudies-literature
| S-EPMC7363046 | biostudies-literature
2010-07-01 | GSE21373 | GEO
| S-EPMC4864301 | biostudies-literature
| S-EPMC6663011 | biostudies-literature