Intra-ovarian injection of bone marrow-derived c-Kit⁺ cells for ovarian rejuvenation in menopausal rats.

ABSTRACT: Introduction: Cell-based therapies with certain cell types are touted as novel and hopeful therapeutic intervention in the clinical setting. Here, we aimed to assess the regenerative potential of c-Kit⁺ cells in the rejuvenation of ovarian tissue and fertility rate in rat model of premature ovarian failure (POF). Methods: Rats were treated with 160 mg/kg/BW of 4-vinylcyclohexene dioxide for 15 days. Freshly enriched rat bone marrow-derived c-Kit⁺ (MACS) and c-Kit^- cells (4×10⁵ cells/10 µL) were transplanted into the ovaries of treatment and control animals. Prior to transplantation as well as 2, 4, 6, and 8 weeks post-transplantation, randomly-selected rats were euthanized and ovarian tissues were subjected to pathop

SUBMITTER: Sheshpari S 

PROVIDER: S-EPMC9376162 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature