Noninvasive imaging of the lung NETosis by anti-Ly6G iron oxide nanoparticles.

ABSTRACT: It is challenging to visualize noninvasively the formation of neutrophil extracellular traps, known as NETosis, and therefore difficult to monitor disease progression. A desirable molecular imaging probe is the iron oxide nanoparticle (NP) that could induce reactive oxygen species. Here, we used C57BL/6 mice with pristane-induced lupus, which mimics systemic lupus erythematosus. Administration of anti-Ly6G antibody-conjugated NP allowed detection of NETosis with fluorescent molecular imaging, as evidenced by flow cytometric analysis of citrullinated histone H3 expression in lung neutrophils. This finding was consistent with NP-induced blood NETosis in a spontaneous lupus model of B6.MRL-lpr mice. A chronic assessment was performed in which the lupus mice were protected from e

SUBMITTER: Zhong J 

PROVIDER: S-EPMC9382280 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature