Successful 3-day lung preservation using a cyclic normothermic ex vivo lung perfusion strategy.

ABSTRACT:

Background

Cold static preservation (CSP) at higher temperatures (10°C) has been recently shown as an optimal strategy up to 24-36h of preservation. Here, we hypothesized that alternating 10°C static storage with cycles of normothermic ex vivo lung perfusion (EVLP) would provide conditions for cellular "recharge", allowing for multi-day lung preservation.

Methods

Donor lungs from male Yorkshire pigs were preserved using 10°C CSP with two cycles of 4h EVLP. After a total of 3 days of preservation, a left lung transplant was performed followed by 4h of graft evaluation. As controls, 2 lungs were preserved solely with continuous 10°C preservation for 3 days and transplanted.

Findings

For animals receiving lungs preserved using a cyclic EVLP protocol, lung function and hi

SUBMITTER: Ali A 

PROVIDER: S-EPMC9385559 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature