Quantitative determination of sn-positional phospholipid isomers in MSⁿ using silver cationization.

ABSTRACT: Glycerophospholipids are one of the fundamental building blocks for life. The acyl chain connectivity to the glycerol backbone constitutes different sn-positional isomers, which have great diversity and importance for biological function. However, to fully realize their impact on function, analytical techniques that can identify and quantify sn-positional isomers in chemically complex biological samples are needed. Here, we utilize silver ion cationization in combination with tandem mass spectrometry (MSⁿ) to identify sn-positional isomers of phosphatidylcholine (PC) species. In particular, a labile carbocation is generated through a neutral loss (NL) of AgH, the dissociation of which provides diagnostic product ions that correspond to acyl chains at the sn-1 or sn-2 position. T

SUBMITTER: Lillja J 

PROVIDER: S-EPMC9482905 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature