Development of a standardized and validated flow cytometry approach for monitoring of innate myeloid immune cells in human blood.

ABSTRACT: Innate myeloid cell (IMC) populations form an essential part of innate immunity. Flow cytometric (FCM) monitoring of IMCs in peripheral blood (PB) has great clinical potential for disease monitoring due to their role in maintenance of tissue homeostasis and ability to sense micro-environmental changes, such as inflammatory processes and tissue damage. However, the lack of standardized and validated approaches has hampered broad clinical implementation. For accurate identification and separation of IMC populations, 62 antibodies against 44 different proteins were evaluated. In multiple rounds of EuroFlow-based design-testing-evaluation-redesign, finally 16 antibodies were selected for their non-redundancy and separation power. Accordingly, two antibody combinations were designed for fast, s

SUBMITTER: van der Pan K 

PROVIDER: S-EPMC9519388 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature