Comparative metagenomic analysis of human intervertebral disc nucleus pulposus and cartilaginous end plates.

ABSTRACT:

Study design

The diversity of microflora inhabiting endplate (EP) and nucleus pulposus (NP) tissues of human intervertebral disc (IVD) was profiled through NGS-supported 16S rRNA amplicon sequencing. Sixteen EP and their corresponding NP were excised from the brain-dead voluntary organ donors with no clinical history of low back pain, and 12 herniated and 8 degenerated NP tissues isolated from the patients undergoing spinal surgery were subjected to study the alteration in the microbial diversity.

Objectives

To understand in normal IVD, whether the colonization of bacteria to the NP is through the EP in discs with intact annulus fibrosus. To identify significantly differing microbial population(s) between normal and diseased IVD (NP).

Background of the study

There is

SUBMITTER: Shanmuganathan R 

PROVIDER: S-EPMC9554234 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature