NaBiF₄ upconversion nanoparticle-based electrochemiluminescent biosensor for E. coli O157 : H7 detection.

ABSTRACT: Foodborne or water-borne pathogens pose great threats to human beings and animals. There is an urgent need to detect pathogens with cheap, rapid and sensitive point-of-care diagnostic assays. Herein, we report the electrochemiluminescent (ECL) behaviors of NaBiF₄ : Yb³⁺/Er³⁺ upconversion nanoparticles (UCNPs) which were synthesized via a fast and environment-friendly method at room temperature for the first time. The UCNPs together with K₂S₂O₈ exhibit high ECL intensity and stable cathodic signals. Further, the Au nanoparticles (Au NPs) and Anti-E. coli O157 : H7 antibody were assembled on the surface of UCNPs successively to construct a novel ECL immunosensor for the detection of deadly E. coli O157 : H

SUBMITTER: Liu D 

PROVIDER: S-EPMC9589827 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature