Quenching of Protein Fluorescence by Fullerenol C₆₀(OH)₃₆ Nanoparticles.

ABSTRACT: The effect of the interaction between fullerenol C₆₀(OH)₃₆ (FUL) and alcohol dehydrogenase (ADH) from Saccharomyces cerevisiae and human serum albumin (HSA) was studied by absorption spectroscopy, fluorescence spectroscopy, and time-resolved fluorescence spectroscopy. As shown in the study, the fluorescence intensities of ADH and HSA at excitation wavelengths λ_ex = 280 nm (Trp, Tyr) and λ_ex = 295 nm (Trp) are decreased with the increase in the FUL concentration. The results of time-resolved measurements indicate that both quenching mechanisms, dynamic and static, are present. The binding constant K_b and the number of binding sites were obtained for HSA and ADH. Thus, the results indicated the formation of FUL complexes and prote

SUBMITTER: Lichota A 

PROVIDER: S-EPMC9603995 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature