Neuroprotective effect of phospholipase A₂ from Malaysian Naja sumatrana venom against H₂O₂-induced cell damage and apoptosis.

ABSTRACT: Oxidative stress is one of the factors involved in the pathogenesis of several neurodegenerative diseases. It has been reported that a secretory phospholipase A₂ known as A2-EPTX-NSm1a has lower cytotoxicity in neuronal cells compared to its crude Naja sumatrana venom. In this study, A2-EPTX-NSm1a was tested for its neuroprotective activity on human neuroblastoma cells (SH-SY5Y) differentiated into cholinergic neurons against oxidative stress induced by hydrogen peroxide (H₂O₂). H₂O₂ treatment alone increased the caspase-3 and caspase-8 activities, whereas pre-treatment with A2-EPTX-NSm1a reduced the activity of these apoptosis-associated proteins. Moreover, A2-EPTX-NSm1a protects the morphology and ultrastructure of differentia

SUBMITTER: Abdullah NAH 

PROVIDER: S-EPMC9614335 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature