Delineating the transcriptional landscape and clonal diversity of virus-specific CD4⁺ T cells during chronic viral infection.

ABSTRACT: Although recent evidence indicates that CD4⁺ T cells responding to chronic viral infection are functionally heterogenous, our understanding of the developmental relationships between these subsets, and a determination of how their transcriptional landscape compares to their acute infection counterparts remains unclear. Additionally, whether cell-intrinsic factors such as TCR usage influence CD4⁺ T cell fate commitment during persistent infection has not previously been studied. Herein, we perform single-cell RNA sequencing (scRNA-seq) combined with single-cell T cell receptor sequencing (scTCR-seq) on virus-specific CD4⁺ T cells isolated from mice infected with chronic lymphocytic choriomeningitis virus (LCMV) infection. We identify several transcriptionall

SUBMITTER: Zander R 

PROVIDER: S-EPMC9629829 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature