Biosynthesis of cannabinoid precursor olivetolic acid in genetically engineered Yarrowia lipolytica.
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ABSTRACT:
SUBMITTER: Ma J
PROVIDER: S-EPMC9653464 | biostudies-literature | 2022 Nov
REPOSITORIES: biostudies-literature
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Biosynthesis of cannabinoid precursor olivetolic acid in genetically engineered Yarrowia lipolytica.
Ma Jingbo J Gu Yang Y Xu Peng P
Communications biology 20221112 1
Engineering microbes to produce plant-derived natural products provides an alternate solution to obtain bioactive products. Here we report a systematic approach to sequentially identify the rate-limiting steps and improve the biosynthesis of the cannabinoid precursor olivetolic acid (OLA) in Yarrowia lipolytica. We find that Pseudomonas sp LvaE encoding a short-chain acyl-CoA synthetase can efficiently convert hexanoic acid to hexanoyl-CoA. The co-expression of the acetyl-CoA carboxylase, the py ...[more]
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