Stabilization and quantitative measurement of nicotinamide adenine dinucleotide in human whole blood using dried blood spot sampling.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺) is a coenzyme essential for energy production. Recently, associations between NAD⁺ and aging-related diseases have been reported, and NAD⁺ precursors that increase NAD⁺ concentration in the body have been acknowledged as anti-aging supplements. However, there have been only a few studies on the link between aging or aging-related diseases and human blood NAD⁺ concentration because NAD⁺ and its precursors are unstable in blood and difficult to measure. Therefore, we aimed to construct a quantitative NAD⁺ measurement method that is simpler than the existing methods. The calibration standards of NAD⁺ showed good linearity (0.9936 to 0.9990) in the range of 0.25 to 2

SUBMITTER: Matsuyama R 

PROVIDER: S-EPMC9741944 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature