Stage-specific requirement for METTL3-dependent m⁶A modification during dental pulp stem cell differentiation.

ABSTRACT:

Background

N⁶-methyladenosine (m⁶A) is the most prevalent epigenetic modification in eukaryotic messenger RNAs and plays a critical role in cell fate transition. However, it remains to be elucidated how m⁶A marks functionally impact the transcriptional cascades that orchestrate stem cell differentiation. The present study focuses on the biological function and mechanism of m⁶A methylation in dental pulp stem cell (DPSC) differentiation.

Methods

m⁶A RNA immunoprecipitation sequencing was utilized to assess the m⁶A-mRNA landscape during DPSC differentiation. Ectopic transplantation of DPSCs in immunodeficient mice was conducted to verify the in vitro findings. RNA sequencing and m⁶A RNA immunoprecipit

SUBMITTER: Luo H 

PROVIDER: S-EPMC9756505 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature