Rapid LC-MS assay for targeted metabolite quantification by serial injection into isocratic gradients.

ABSTRACT: Liquid chromatography mass spectrometry (LC-MS) has emerged as a mainstream strategy for metabolomics analyses. One advantage of LC-MS is that it can serve both as a biomarker discovery tool and as a platform for clinical diagnostics. Consequently, it offers an exciting opportunity to potentially transition research studies into real-world clinical tools. One important distinction between research versus diagnostics-based applications of LC-MS is throughput. Clinical LC-MS must enable quantitative analyses of target molecules in hundreds or thousands of samples each day. Currently, the throughput of these clinical applications is limited by the chromatographic gradient lengths, which-when analyzing complex metabolomics samples-are difficult to conduct in under ~ 3 min per sample without in

SUBMITTER: Groves RA 

PROVIDER: S-EPMC9823034 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature