Unknown

Dataset Information

0

Characterization of a Conjugative Hybrid Plasmid Coharboring blaKPC-2 and blaIMP-4 in a Klebsiella quasipneumoniae Clinical Isolate.


ABSTRACT: Generation of hybrid MDR plasmids accelerated the evolution and transmission of resistance genes. In this study, we characterized a blaKPC-2- and blaIMP-4-coharboring conjugative hybrid plasmid constituted of an IncHI5 plasmid-like region, an IncFII(Yp)/IncFIA plasmid-like region, and a KPN1344 chromosome-like region from a clinical ST852-KL18 Klebsiella quasipneumoniae strain. The blaIMP-4 gene was captured by a novel integron In1965, and the blaKPC-2 gene was located on a new non-Tn4401 group I NTEKPC element. Both blaKPC-2- and blaIMP-4-containing genetic architectures were distinguished from classical structures, highlighting the constant evolution of these genetic elements. IMPORTANCE The emergence of carbapenem-resistant Enterobacterales (CRE) that coexpress serine- and metallo-carbapenemases is a severe threat to the efficacy of ceftazidime-avibactam (CZA), which has been proven to be extremely effective against KPC-producing Enterobacterales strains. Our study described the cooccurrence of KPC-2, a serine β-lactamase, and IMP-4, a metallo-β-lactamase (MBL), on a conjugative hybrid plasmid from a clinical carbapenem-resistant K. quasipneumoniae strain, and it revealed an alternative route for IncHI5 plasmid to evolve by recombining with other plasmids to form a hybrid plasmid. Moreover, this hybrid plasmid can be transferred into other Klebsiella species and stably persist during passage. The propagation of two important carbapenemase genes with a new genetic background using well-evolved plasmids in the clinical setting promotes the emergence of superbugs that require careful monitoring.

SUBMITTER: Dong H 

PROVIDER: S-EPMC9927271 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterization of a Conjugative Hybrid Plasmid Coharboring <i>bla</i><sub>KPC-2</sub> and <i>bla</i><sub>IMP-4</sub> in a Klebsiella quasipneumoniae Clinical Isolate.

Dong Huiyue H   Liu Ziyi Z   Wu Zhiyao Z   Zhang Tingting T   Xia Ziwei Z   Zhao Yuxin Y   Li Yan Y   Shi Jinjin J   Wang Zhiqiang Z   Li Ruichao R   Qin Shangshang S  

Microbiology spectrum 20230110 1


Generation of hybrid MDR plasmids accelerated the evolution and transmission of resistance genes. In this study, we characterized a <i>bla</i><sub>KPC-2</sub>- and <i>bla</i><sub>IMP-4</sub>-coharboring conjugative hybrid plasmid constituted of an IncHI5 plasmid-like region, an IncFII(Yp)/IncFIA plasmid-like region, and a KPN1344 chromosome-like region from a clinical ST852-KL18 Klebsiella quasipneumoniae strain. The <i>bla</i><sub>IMP-4</sub> gene was captured by a novel integron In<i>1965</i>,  ...[more]

Similar Datasets

| S-EPMC7012882 | biostudies-literature
| S-EPMC6923860 | biostudies-literature
| S-EPMC7546619 | biostudies-literature
| S-EPMC10525660 | biostudies-literature
| S-EPMC11022923 | biostudies-literature
| S-EPMC8923210 | biostudies-literature
| S-EPMC10100677 | biostudies-literature
| S-EPMC11535437 | biostudies-literature
| S-EPMC10918965 | biostudies-literature