Separation of Isomeric Forms of Urolithin Glucuronides Using Supercritical Fluid Chromatography.

ABSTRACT: Urolithins are gut microbiota metabolites produced in humans after consuming foods containing ellagitannins and ellagic acid. Three urolithin metabotypes have been reported for different individuals depending on the final urolithins produced. After absorption, they are conjugated with glucuronic acid (phase II metabolism), and these are the main circulating metabolites in plasma and reach different tissues. Different regioisomeric isomers of urolithin glucuronides have been described. Still, their identification and quantification in humans have not been properly reported due to resolution limitations in their analysis by reversed-phase high-performance liquid chromatography. In the present study, we report a novel method for separating these isomers using supercritical fluid chromatograph

SUBMITTER: Ares AM 

PROVIDER: S-EPMC9936581 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature