Ultrasound-pretreatment combined with Ti3C2-TiO2-AuNPs enhancing the electrogenerated chemiluminescence of the air-saturated luminol for exosomes detection

ABSTRACT: Graphical abstract The cavitation effect of high-intensity focused ultrasound could generate the H2O2 and O2–• in situ. Ti3C2-TiO2-AuNPs absorbed and catalyzed H2O2 to produce more O2–•. The synergistic effect leads to a great enhancement of the ECL emission for the air-saturated luminol. Highlights • High-intensity focused ultrasound triggered the ECL of the air-saturated luminol.• A novel probe of Ti3C2-TiO2-AuNPs was synthetized.• The Ti3C2-TiO2-AuNPs could absorb and catalyze the H2O2 to O2–•.• Through combination of ultrasound and probe, the exosomes were detected sensitively. It is still a great challenge to develop effective strategies to improve the low electrogenerated chemiluminescence (ECL) of air-saturated luminol. Herein, the synergistic effects of Ti3C2-TiO2-AuNPs nano hybrid and high-intensity focused ultrasound pretreatment (ultrasound-pretreatment) were used to significantly improve the ECL emission of the air-saturated luminol, and the mechanism was proposed. The ultrasound-pretreatment as a green method with the cavitation effect could form O2–• and H2O2 in situ as an initiator. TiO2 and Au nanoparticles (AuNPs) were in situ decorated on the Ti3C2 surface to form Ti3C2-TiO2-AuNPs, and it was proved as a highly efficient booster which could catalyze and aggregate H2O2 to the O2–•. The utilization rate of intermediates has been greatly improved. Exosomes as model targets can be sensitively detected by the ECL sensor. The detection limit was 195 particles μL−1. The detection results of exosomes in actual samples are satisfactory. We believe that the ultrasound-pretreatment strategy could be extended to the sensitive detection in the biological sample.

SUBMITTER:  

PROVIDER: S-EPMC9969320 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature