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Genes Differentially Expressed as a Result of Enforced Pokemon Expression in MCF-7 cell

ABSTRACT: Pokmeon is an oncogenic transcription factor involved in cell growth, differentiation, and oncogenesis,but its regulatory network in breast cancer cells remians elusive . We used microarray to determine the down stream target genes of pokemon by using the MCF-7 cell line with stable ectopic expression of pokemon. The MCF-7 cell clones with high ectopic expression of pokemon were screened by the Hygromycin B,and the control cell line was also obtained by introducing empty vector .Both the RNA were extracted and hybridized with 22k cDNA spots from CapitalBio, Chin

ORGANISM(S): Homo sapiens

SUBMITTER: Zu Xuyu

PROVIDER: S-ECPF-GEOD-27442 | biostudies-other |

REPOSITORIES: biostudies-other

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Genes Differentially Expressed as a Result of Enforced Pokemon Expression in MCF-7 cell

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Genes Differentially Expressed as a Result of Enforced Pokemon Expression in MCF-7 cell

Project description:Pokmeon is an oncogenic transcription factor involved in cell growth, differentiation, and oncogenesis,but its regulatory network in breast cancer cells remians elusive . We used microarray to determine the down stream target genes of pokemon by using the MCF-7 cell line with stable ectopic expression of pokemon. The MCF-7 cell clones with high ectopic expression of pokemon were screened by the Hygromycin B,and the control cell line was also obtained by introducing empty vector .Both the RNA were extracted and hybridized with 22k cDNA spots from CapitalBio, Chin

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Genes differentially expressed as a result of Candida albicans SC5314 exposure to pterostilbene

Project description:Transcriptional profiling of Candida albicans comparing pterostilbene treated cells with untreated cells

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Genes Differentially Expressed as a result of Candida albicans SC5314 exposure to 10b

Project description:Azole resistance and varying degrees of cross-resistance to other members of the azole family in clinical isolates have been documented, which has necessitated additional and prolonged use of the antifungal agents available. 2-Amino-Nonyl-6-Methoxyl-Tetralin Muriate (10b), a novel chemical structural aminotetralin derivate, is synthesized as an antifungal agent and exibited strong antifungal activity. To further investigated the action mechanism, we used microarray analysis to investigate the genes expression profiles of C. albicans cells treated or untreated with 10b and found 957 genes were differentially expressed. Of them,457 showed a decrease in expression and 500 showed an increase in expression. 33 down-regulated genes were involved in glycolysis (e.g., PFK1, CDC19 and HXK2), fermentation (e.g., PDC11, ALD5 and ADH1) and respiratory electron transport chain (e.g., CBP3, COR1 and QCR8). 30 differentially expressed genes were found to relate to biofilm formation, filamentous or hyphal growth. It was noticed that striking up-regulation of SFL1 and marked down-regulation of YWP1 directly related to prevent C. albicans from changing its morphology from the yeast form to the hyphal. Two genes related to specifically hydrolyzing beta-1, 3 glucan (e.g., XOG1) and chitin (e.g., CHT1) were significantly increased. 40 overexpressed genes and 15 down-regulated genes were related to the lipid metabolic process. Of them, Eight were directly linked to ergosterol biosynthesis, including ERG2, ERG6 and ERG11. 99 genes related to translation were down-regulated following exposure to 10b, which account for 21.66% in down-regulated genes. This suggested that translation might be lower in SC5314 cells exposed to 10b than in control.

2011-05-11 | GSE19552 | GEO

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