Ontology highlight
ABSTRACT:
SUBMITTER: Nandal A
PROVIDER: S-EPMC3361910 | biostudies-other | 2011 Nov
REPOSITORIES: biostudies-other
Nandal Anjali A Ruiz Julio C JC Subramanian Poorna P Ghimire-Rijal Sudipa S Sinnamon Ruth Ann RA Stemmler Timothy L TL Bruick Richard K RK Philpott Caroline C CC
Cell metabolism 20111101 5
Mammalian cells express dozens of iron-containing proteins, yet little is known about the mechanism of metal ligand incorporation. Human poly (rC) binding protein 1 (PCBP1) is an iron chaperone that binds iron and delivers it to ferritin, a cytosolic iron storage protein. We have identified the iron-dependent prolyl hydroxylases (PHDs) and asparaginyl hydroxylase (FIH1) that modify hypoxia-inducible factor α (HIFα) as targets of PCBP1. Depletion of PCBP1 or PCBP2 in cells led to loss of PHD acti ...[more]