Single-cell sequencing of developing human gut reveals transcriptional links to childhood Crohn’s disease

ABSTRACT: In this study, we performed single-cell transcriptional profiling of human embryonic and fetal gut samples obtained from 9 human embryos spanning ages 6-10 PCW and three regions (duo-jejunum, ileum and colon). Additionally, we profile mucosal biopsies from the terminal ileum of healthy children aged 4-12 years (n = 8) as well as a group of children newly diagnosed with Crohn’s disease (CD) (n = 7), a common form of IBD. Tissue samples were treated using an enzymatic dissociation protocol and single cell suspensions were then processed using the 3’v2 10x Genomics Chromium workflow. In a subset of samples, the intestinal epithelial cell fraction was enriched by performing magnetic bead sorting for EPCAM. In total, we generate single cell transcriptomes of ~90,000 primary human intestinal cel

SUBMITTER: Rasa Elmentaite 

PROVIDER: S-SUBS9 | biostudies-other |

SECONDARY ACCESSION(S): SAMEA6877938

REPOSITORIES: biostudies-other