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Differential Gene Expression Analysis of Human Cumulus Cells as a Predictor of Pregnancy

ABSTRACT: The objective of this study was to provide valuable insights into the determination of clinical pregnancy and live birth outcomes in the context of differential gene expression analysis of cumulus cells as predictors of clinical pregnancy and/or live birth after single embryo transfers. This study was performed with the consideration that each oocyte and each group of cumulus cells surrounding different oocytes might have different genetic expression patterns that might affect human reproduction. Differential gene expression analysis of cumulus cells was performed in 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes of 10 patients. Same procedures related to the oocyte maturation, microinjection, and microarray analyses were applied to the group of cumulus cells, individually. The results of the microarray analyses were enriched according to the clinical pregnancy and live birth outcomes of the patients. In conclusion, our data indicated significant differences in gene expression related with a variety of signaling pathways; the RAS signaling, the mitogen-activated protein kinases signalling, the ERBB signaling, the RAP1 signaling, the transcription factor nuclear factor-kb signaling, the transforming growth factor-b signaling pathways and neurological signaling pathways such as glutamatergic synapse, cholinergic synapse, dopaminergic synapse, GABAergic synapse, long-term potentiation, the neuroactive ligand-receptor interaction pathways. Circadian entrainment pathway was also affected in both clinical pregnancy and live birth at different significance level. These pathways can be exploited to identify biomarkers related to clinical pregnancy and live birth outcomes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE113239 | GEO | 2018/04/18

REPOSITORIES: GEO

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