Transcriptomics,Genomics

Dataset Information

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Gene expression profiling of prostate stromal cells at proximal and distal ducts


ABSTRACT: We carried out gene expression profiling of FACS-isolated prostate stromal cells at adult mouse proximal ducts and at distal ducts. We found that proximal stromal cells possess higher Wnt activity. We confirmed that TGFβ2 is highly expressed in FACS-isolated stromal cells at adult mouse proximal ducts than in those at distal ducts, whereas TGFβ3 expression is slightly higher but not statistically significant. These observations suggest that TGFβ2 and TGFβ3 may be regulated by Wnt signaling in prostate stromal cells. Overall design: Two group comparison

INSTRUMENT(S): Illumina Genome Analyzer (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Chad Creighton  

PROVIDER: GSE115631 | GEO | 2019-06-21

REPOSITORIES: GEO

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Publications

Spatially Restricted Stromal Wnt Signaling Restrains Prostate Epithelial Progenitor Growth through Direct and Indirect Mechanisms.

Wei Xing X   Zhang Li L   Zhou Zhicheng Z   Kwon Oh-Joon OJ   Zhang Yiqun Y   Nguyen Hoang H   Dumpit Ruth R   True Lawrence L   Nelson Peter P   Dong Baijun B   Xue Wei W   Birchmeier Walter W   Taketo Makoto M MM   Xu Feng F   Creighton Chad J CJ   Ittmann Michael M MM   Xin Li L  

Cell stem cell 20190411 5


Cell-autonomous Wnt signaling has well-characterized functions in controlling stem cell activity, including in the prostate. While niche cells secrete Wnt ligands, the effects of Wnt signaling in niche cells per se are less understood. Here, we show that stromal cells in the proximal prostatic duct near the urethra, a mouse prostate stem cell niche, not only produce multiple Wnt ligands but also exhibit strong Wnt/β-catenin activity. The non-canonical Wnt ligand Wnt5a, secreted by proximal strom  ...[more]