Project description:To investigate the candidate molecular mechanisms underlies methamphetamine-associated CPP memory retrieval and reconsolidation, we compared the transcriptional profling changes in the mPFC between mice that underwent retrieval and non-retrieval after methamphetamine-paired CPP training.
Project description:Hypothalamic hamartomas (HHs) are congenital lesions of the neuroendocrine brain composed of neurons and astroglia. Frequently, HHs are associated with central precocious puberty (CPP) and/or gelastic seizures. Because HHs might express genes similar to those required for the initiation of normal puberty we used cDNA arrays to compare the gene expression profile of a HH associated with CPP with three HHs not accompanied by sexual precocity. Our aim was to identify genes whose expression may be selectively altered in the HH with CPP and hence, involved in the onset of puberty. Keywords: comparison between HH with and without CPP
Project description:Hypothalamic hamartomas (HHs) are congenital lesions of the neuroendocrine brain composed of neurons and astroglia. Frequently, HHs are associated with central precocious puberty (CPP) and/or gelastic seizures. Because HHs might express genes similar to those required for the initiation of normal puberty we used cDNA arrays to compare the gene expression profile of a HH associated with CPP with three HHs not accompanied by sexual precocity. Our aim was to identify genes whose expression may be selectively altered in the HH with CPP and hence, involved in the onset of puberty. Experiment Overall Design: Affymetrix arrays were used to detect global changes in gene expression. The results of this analysis were confirmed by semi-quantitative PCR.
Project description:In order to screen for molecules contributing to the effects of FGF1/CPP-C on radiation-induced intestinal damage, the total RNA expression profile of the irradiated jejunum was obtained using a DNA microarray analysis after Carbon-ion irradiation with the FGF1/CPP-C pretreatment.
Project description:Chemical Denaturation and Protein Precipitation (CPP) was performed on an E. coli lysate in order to determine the precipitaiton midpoint for the proteome
Project description:Analysis by LC-MS/MS shotgun proteomics was performed on lysates from the following samples: 1) Control HK2 cells generated using control CRISPR/CAS9 system and scrambled guide RNA (sgRNAGCACTCACATCGCTACATCA) 2) HK2 cells with FTO knockout using CRISPR/Cas9 system and target 2 guide RNA (sgCCTACAAGTACCTGAACACC) 3) HK2 cells with FTO knockout using CRISPR/Cas9 system and target 3 guide RNA (sgCCAGGCTCTTTACGGTCCCC) 4) HK2 cells treated with vehicle control 5) HK2 cells treated with erastin 6) HK2 cells treated with FTO inhibitor FB23-2
