Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LPS effects on macrophages

ABSTRACT: RAW264.7 murine macrophages treated with LPS Keywords: time-course

ORGANISM(S): Mus musculus

PROVIDER: GSE1459 | GEO | 2004/06/05

SECONDARY ACCESSION(S): PRJNA90013

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of mouse macrophage response to LPS

Project description:RAW264.7 murine macrophages treated with LPS

2008-06-11 | E-GEOD-1459 | biostudies-arrayexpress

LPS induced gene expression in macrophages in the presence of Carbon monoxide

Project description:Murine RAW 264.7 peritoneal macrophages were treated with 250 ppm Carbon monoxide (CO) for 3 hours prior to the addition of LPS (10 ng/ml) and then for the subsequent 4 hours of the study. Air-treated cells were maintained for the same time without CO. Total RNA was harvested at 0, 15, 30, 60, 120 and 240 min after LPS addition. CRNA was produced using standard Affymetrix procedures from 10µg of total RNA. The expression values of all probe sets are scaled to a target intensity of 500 by using Affymetrix Microarray Suite 5.0 Keywords = macrophage Keywords = inflammation Keywords = endotoxin Keywords = gene expression Keywords: time-course

2004-11-05 | GSE1906 | GEO

A quantitative model for macrophage activation predicts tunable distance thresholds for propagation of inflammationes in response LPS stimulation

Project description:Label free analysis of the secretome of RAW264.7 murine macrophages in response to LPS stimulation.

2015-11-30 | PXD001905 | Pride

Time course of PBL derived avian macrophages stimulated with whole Ecoli or LPS

Project description:This series compares gene expression in chicken peripheral blood lymphocyte (PBL) derived macrophages following stimulation with either XL10 E. coli or S. typh LPS over an 8 hour time course (0,1,2,4,and 8 hours). For the late time points, the stimuli were removed after 2 hours and fresh media was added for the remaining time. Keywords: time-course

2004-09-24 | GSE1794 | GEO

H3K4methylation in Setd1a loss-of-function Raw264.7 cells upon LPS or Dex+LPS stimulation

Project description:Nuclear interaction studies by ChIP coupled with mass spectrometry identified the COMPASS/SETD1A complex as interaction partner of the glucocorticoid receptor (GR) in murine bone marrow-derived macrophages (BMDMs). Here, we profiled H3K4me1, H3K4me2 and H3K4me3 in wild-type and Setd1a hypermorphic (Setd1aDel/+) Raw264.7 cells after LPS and Dex+LPS stimulation by spike-in ChIP-Seq.

2020-12-31 | GSE138017 | GEO

Time-series of human LPS stimulated-monocyte derived macrophages

Project description:We studied the lipidome (236 individual lipid species including sphingolipids, ceramides, cholesterol, gangliosides, phosphatidylethanolamines) in basal and LPS-stimulated human monocyte derived macrophages (MDMs) over a time-course by LC-MS (30min, 3h, 8h, 16h; n=12 human donors). In order to explore how transcriptomic changes induced by LPS stimulation can correlate with changes in the lipidome, we performed RNAsequencing on MDMs from 4 donors over a time-course (30min, 3h, 8h, 16h)

2022-08-02 | GSE112372 | GEO

Setd1a and Setd1b loss-of-function in murine macrophages

Project description:Setd1bKO primary murine bone marrow-derived macrophages (BMDMs) were treated with lipopolysaccharide (LPS) or dexamethasone and LPS (Dex+LPS) and gene expression differences in response to treatment analysed by PolyA RNA-Sequencing. No Dex-treatment dependent gene expression differences were identified. Setd1aDel/+ Raw264.7 cells with reduced Setd1a expression were analyzed with regards to their reponse to Dex when inflammatorily challenged with LPS by mRNA-Seq. We observed reduced GR-dependent gene acivation in Setd1a hypermorphic Raw264.7 cells. Wild type and Setd1aDel/+ Raw264.7 cells were treated with LPS or LPS and interferon beta (IFNB1) to show the IFNB1-dependent loss of gene expression in LPS-stimulated Setd1aDel/+ cells.

2020-12-31 | GSE137944 | GEO

Macrophages Solvent, AA, LPS, AA+LPS

Project description:Macrophages Solvent, AA, LPS, AA+LPS

| PRJEB47005 | ENA

Analysis of cytoplasmic, nucleoplasmic, nucleolar transcriptome in Raw264.7 macrophages during LPS stimulation

Project description:To understand a novel function of nucleolus during infectious conditions, we purified cytoplasmic, nucleoplasmic, and nucleolar fractions from macrophages for a time course of LPS stimulation and performed RNA-seq.

2020-10-13 | GSE159346 | GEO

HuR modulation impairs LPS-response in murine macrophages

Project description:Lipopolysaccharide exposure to macrophages induces an inflammatory response that is heavily regulated at the post-transcriptional level. HuR, ELAVL1, is an RNA binding protein that binds and regulate the maturation and half-life of AU/U rich elements (ARE) containing cytokines and chemokines transcripts, mediating the LPS induced response. Here we investigated to what extent small molecules inhibiting HuR-RNA interaction, called tanshinone mimics, counteract the LPS induced macrophage response. We show that tanshinone mimics are present in solution in a keto-enolic tautomerism and that, by molecular dynamic calculations, the ortho quinone form is the preferred species interacting with HuR and favoring the closure of its conformation to a no-binding mode. The protection of the enolic status with diacetate caused the loss of activity of tanshinone mimics in vitro but active in vivo. Murine macrophages cell line RAW264.7 was treated with LPS and tanshinone mimics and the modulation of the LPS induced response was monitored by RNA and Ribonucleoprotein immunoprecipitation sequencing. Correlation analyses indicated that LPS induced a strong coupling between differentially expressed genes and HuR-bound genes and that tanshinone mimics reduced the interaction. Functional annotation addressed a specific set of genes, as Cxcl10, Il1b, Cd40, Fas, involved in chemotaxis and immune response whose association with HuR decreased and led to a reduction of their protein level and secretion. The same effect was observed in primary murine bone marrow derived macrophages and in vivo in a LPS induced peritonitis model, in which the serum level of Cxcl10 and Il1b was strongly reduced, endowing tanshinone mimics with anti-inflammatory properties in vivo.

2023-03-01 | GSE198605 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data