Transcriptomics

Dataset Information

28

The effect of ERG overexpression and ERG knockdown by shRNA in RWPE1 and VCAP cells, respectively


ABSTRACT: The goal of this project was to analyze the global gene expression profiles of RWPE1 and VCAP cells following transfection of GFP, GFP-ERG at 48 and 72hrs time points and stable ERG shRNA, scramble shRNA, respectively. Overall design: RWPE1 cells were transfected with GFP or GFP-ERG. VCAP cells were transfected with ERG lenti-shRNA or scramble shRNA. Transfections were performed in duplicate. Total cellular RNA was isolated with Trizol and quality analysed by the bioanalyser kit.

INSTRUMENT(S): Illumina humanRef-8 v2.0 expression beadchip

ORGANISM(S): Homo sapiens  

SUBMITTER: Santosh Kumar Gupta  

PROVIDER: GSE16671 | GEO | 2010-08-26

SECONDARY ACCESSION(S): PRJNA117385

REPOSITORIES: GEO

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