Transcriptomics,Genomics

Dataset Information

28

Potential mechanisms of low salt diet induced cardiac diseases


ABSTRACT: We hypothesized that a LS intake leads to a cascade of events involving increases in plasma AII, the AT1 receptor, NADPH oxidase and O2˙ production and ultimately to reduced NO bioactivity. This reduced NO bioactivity results in altered control of the coronary circulation and cardiac metabolism. This study was undertaken to determine the global changes in cardiac gene expression in dogs fed LS diet (0.5% NaCl) for two weeks. Cardiac RNA was extracted from the control and LS fed groups (n=4). Statistical significance (P≤0.05; fold change of ±2) showed that there were 443 genes that changed, 110 decreasing and 333 increasing. All of the differentially expressed genes were grouped into functional categories based on their Gene Ontology. Overall design: Adult male mongrel dogs (n=10, BW;25-28kg) were put on LS which contains 0.5% sodium chloride for two weeks. RNA was isolated using Trizol reagent. RNA from each sample was used to generate a high fidelity cDNA using the labeling protocols for sample preparation recommended by Affymetrix. Data and statistical analysis were performed in Genetraffic using a t-test and with variance stabilization. Differences were considered statistically significant at a nominal significance of p≤0.05 and at least ±2 fold change in expression between control (n=3), and LS dogs (n=4).

INSTRUMENT(S): [Canine_2] Affymetrix Canine Genome 2.0 Array

SUBMITTER: Caroline Ojaimi  

PROVIDER: GSE17149 | GEO | 2009-12-22

SECONDARY ACCESSION(S): PRJNA119745

REPOSITORIES: GEO

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Publications

Potential mechanisms of low-sodium diet-induced cardiac disease: superoxide-NO in the heart.

Suematsu Nobuhiro N   Ojaimi Caroline C   Recchia Fabio A FA   Wang Zipping Z   Skayian Yester Y   Xu Xiaobin X   Zhang Suhua S   Kaminski Pawel M PM   Sun Dong D   Wolin Michael S MS   Kaley Gabor G   Hintze Thomas H TH  

Circulation research 20091210 3


Patients on a low salt (LS) diet have increased mortality.To determine whether reduction in NO bioactivity may contribute to the LS-induced cardiac dysfunction and mortality.Adult male mongrel dogs were placed on LS (0.05% sodium chloride) for 2 weeks. Body weight (25.4 + or - 0.4 to 23.6 + or - 0.4 kg), left ventricular systolic pressure (137.0 + or - 3.4 to 124.0 + or - 6.7 mm Hg), and mean aortic pressure (111 + or - 3.1 to 98 + or - 4.3 mm Hg) decreased. Plasma angiotensin II concentration i  ...[more]

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