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Transcriptome of U251-APP cells with CTCF-binding sites knockout

ABSTRACT: Two CTCF-binding sites (CTCF-A and CTCF-B) might mediate frequent chromatin interactions in the 11p11.2 locus and contribute to the co-regulation of genes within the 11p11.2 locus. In order to verify whether knockout of these CTCF-binding sites could influence expression of genes in the 11p11.2 locus, and explore the subsequent consequences of the knockout, we knocked out two CTCF-binding sites (CTCF-A and CTCF-B) in U251 cell with a stable expression of mutant APP 128 (M671L) (U251-APP cells). RNA-seq (by IlluminaHiseq 4000) and following analyses were then performed to detect genes whose expression were influenced by CTCF-binding sites knockout.

ORGANISM(S): Homo sapiens

PROVIDER: GSE173328 | GEO | 2025/12/31

REPOSITORIES: GEO

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