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Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [RNA-seq]


ABSTRACT: To describe the changes of transcriptome in Bend2 knockout mice, we conducted RNA-seq analyses by using leptotene and zygotene spermatocytes from Bend2 WT and KO mice. We found BEND2 contributes to shutting down the mitotic program and to activating or enhancing the meiotic and post-meiotic program of spermatogenic cells. Overall design: Leptotene and zygotene spermatocytes isolated by FACS from Bend2 WT and KO mice were performed in each experiment. RNA-seq data analyses were based on three independent replications.

INSTRUMENT(S): Illumina NovaSeq 6000 (Mus musculus)

ORGANISM(S): Mus Musculus

SUBMITTER: Chunsheng Han  

PROVIDER: GSE189598 | GEO | 2022-06-01

REPOSITORIES: GEO

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Identification and characterization of BEND2 as a key regulator of meiosis during mouse spermatogenesis.

Ma Longfei L   Xie Dan D   Luo Mengcheng M   Lin Xiwen X   Nie Hengyu H   Chen Jian J   Gao Chenxu C   Duo Shuguang S   Han Chunsheng C  

Science advances 20220525 21


The chromatin state, which undergoes global changes during spermatogenesis, is critical to meiotic initiation and progression. However, the key regulators involved and the underlying molecular mechanisms remain to be uncovered. Here, we report that mouse BEND2 is specifically expressed in spermatogenic cells around meiotic initiation and that it plays an essential role in meiotic progression. <i>Bend2</i> gene knockout in male mice arrested meiosis at the transition from zygonema to pachynema, d  ...[more]

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