Transcriptomics,Genomics

Dataset Information

18

The effect of exogenous strigolactone on gene expression in Arabidopsis seedlings


ABSTRACT: Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed microarray analysis to understand the molecular mechanisms underlying SL signaling. Overall design: We performed microarray analysis using strigolactone (SL)-deficient mutants to compare gene expression profiles in response to GR24 to determine whether exogenous applied SL would modulate gene expression. We used cRNAs prepared from max3 seedlings treated with GR24 (1 uM) or DMSO (mock) for 90 min. Two independent analyses were performed, including seedling preparation.

INSTRUMENT(S): [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array

SUBMITTER: kiyoshi mashiguchi  

PROVIDER: GSE19661 | GEO | 2009-12-28

SECONDARY ACCESSION(S): PRJNA122563

REPOSITORIES: GEO

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Publications

Feedback-regulation of strigolactone biosynthetic genes and strigolactone-regulated genes in Arabidopsis.

Mashiguchi Kiyoshi K   Sasaki Eriko E   Shimada Yukihisa Y   Nagae Miyu M   Ueno Kotomi K   Nakano Takeshi T   Yoneyama Koichi K   Suzuki Yoshihito Y   Asami Tadao T  

Bioscience, biotechnology, and biochemistry 20091107 11


Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) and microarray analysis using wild-type plants and SL-deficient/insensitive mutants to understand the molecular mechanisms underlying SL biosynthesis and signaling. We found that there is responsiveness to SL in  ...[more]

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