Transcriptomics,Genomics

Dataset Information

49

Gene Expression Profiling of C2C12 Myoblast Differentiation


ABSTRACT: In this study, the C2C12 cell line, a model used to study myogenesis and regeneration, was allowed to differentiate from myoblast precursor cells to myotubes. Cells were harvested at 4 different timepoints to perform gene expression profiling. We identified genes that were up-regulated and down-regulated during the differentiation process. Overall design: C2C12 Myoblasts were allowed to differentiate into myotubes. Cells at different timepoints were harvested for gene expression profiling. Cells from the myoblasts stage, the start of differentiation, 24h after the start of differentiation and myotubes (96h after start of differentiation) were harvested. Myotubes were detatched from the undifferentiated myoblast reserve cells using diluted trypsin. 3 independent biological replicates per timepoint were submitted to gene expression profiling.

INSTRUMENT(S): Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)

SUBMITTER: Alexandre Blais 

PROVIDER: GSE19968 | GEO | 2010-07-05

SECONDARY ACCESSION(S): PRJNA123703

REPOSITORIES: GEO

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Publications

Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation.

Liu Yubing Y   Chu Alphonse A   Chakroun Imane I   Islam Uzma U   Blais Alexandre A  

Nucleic acids research 20100702 20


Precise regulation of gene expression is crucial to myogenesis and is thought to require the cooperation of various transcription factors. On the basis of a bioinformatic analysis of gene regulatory sequences, we hypothesized that myogenic regulatory factors (MRFs), key regulators of skeletal myogenesis, cooperate with members of the SIX family of transcription factors, known to play important roles during embryonic skeletal myogenesis. To this day little is known regarding the exact molecular m  ...[more]

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