Dataset Information


Genomic mapping of Six1 binding sites during skeletal myoblast differentiation

ABSTRACT: In this study, the C2C12 cell line, a model used to study myogenesis and regeneration, was allowed to differentiate from myoblast precursor cells to myotubes. Cells were harvested at 3 different timepoints to perform ChIP-on-Chip of Six1, which is a key muscle regulator. We identified global loci bound by Six1 during skeletal myoblast differentiation. Overall design: C2C12 Myoblasts were allowed to differentiate into myotubes. Cells at three timepoints were harvested for ChIP-on-Chip, including myoblasts stage, 24h after differentiation and myotubes (96h after differentiation). Myotubes were detached from the undifferentiated myoblast reserve cells using diluted trypsin. 3 independent biological replicates were used for each time point experiment. A microarray set counts 3 arrays (Custom Arrays A, B and C) for a total of approximately 2.9 million probes.

INSTRUMENT(S): Agilent-022328 Six_ChIP_01_of_03_v2

ORGANISM(S): Mus musculus  

SUBMITTER: Alexandre Blais 

PROVIDER: GSE20150 | GEO | 2010-07-05



Similar Datasets

2010-07-05 | E-GEOD-20150 | ArrayExpress
| GSE19968 | GEO
2016-08-08 | E-GEOD-73704 | ArrayExpress
2018-07-11 | GSE102614 | GEO
| GSE19967 | GEO
2011-12-08 | E-GEOD-34238 | ArrayExpress
2010-07-30 | E-GEOD-19967 | ArrayExpress
2017-01-02 | PXD004960 | Pride
2011-12-08 | GSE34238 | GEO
| GSE63051 | GEO