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Dataset Information

41

Genomic mapping of Six1 binding sites during skeletal myoblast differentiation


ABSTRACT: In this study, the C2C12 cell line, a model used to study myogenesis and regeneration, was allowed to differentiate from myoblast precursor cells to myotubes. Cells were harvested at 3 different timepoints to perform ChIP-on-Chip of Six1, which is a key muscle regulator. We identified global loci bound by Six1 during skeletal myoblast differentiation. Overall design: C2C12 Myoblasts were allowed to differentiate into myotubes. Cells at three timepoints were harvested for ChIP-on-Chip, including myoblasts stage, 24h after differentiation and myotubes (96h after differentiation). Myotubes were detached from the undifferentiated myoblast reserve cells using diluted trypsin. 3 independent biological replicates were used for each time point experiment. A microarray set counts 3 arrays (Custom Arrays A, B and C) for a total of approximately 2.9 million probes.

INSTRUMENT(S): Agilent-022328 Six_ChIP_01_of_03_v2

ORGANISM(S): Mus musculus  

SUBMITTER: Alexandre Blais 

PROVIDER: GSE20150 | GEO | 2010-07-05

SECONDARY ACCESSION(S): PRJNA125575

REPOSITORIES: GEO

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