Genomics

Dataset Information

39

Livestock-associated MRSA lack accessory virulence determinants of human-associated MRSA


ABSTRACT: Previous studies have documented the diversity of genetic background of methicillin-resistant S. aureus (MRSA) strains associated with healthcare (HA-MRSA), community (CA-MRSA) and livestock (LA-MRSA). The accessory and core-variable genome content of those strains remain largely unknown. To compare the composition of accessory and core-variable genome of Belgian MRSA strains according to host, population setting and genetic background, representative strains of HA- (n=21), CA- (n = 13) and ST398 LA-MRSA (n = 18) were characterized by a DNA-microarray (StaphVar Array) composed of oligonucleotide probes targeting ~400 resistance, adhesion and virulence associated genes.ST398 strains displayed very homogenous hybridization profiles (>94% gene content homology) irrespective of their host origin. This “ST398-specific” genomic profile was not distantly demarked from those of certain human-associated lineages but lacked several virulence- and colonization-associated genes harbored by strains of human origin, such as genes encoding proteases, haemolysins or adhesins. No enterotoxin gene was found among ST398 strains. In conclusion, our findings are consistent with a non-human origin of this ST398 lineage but suggest that it might have the potential to adapt further to the human host. Overall design: CGH microarray was performed on epidemiologically distinct human and animal isolates of methicillin resistant S.aureus. S. aureus labeled genomic DNA were hybridized to StaphVar arrays containing 1326 60mer oligonucleotide probes (Eurogentec, Belgium).

INSTRUMENT(S): ULB-Erasme_MGE_Staphaureus_v2.00

SUBMITTER: Ricardo Luis De Mendonca  

PROVIDER: GSE20541 | GEO | 2010-11-15

SECONDARY ACCESSION(S): PRJNA125295

REPOSITORIES: GEO

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Publications

Diversity of accessory genome of human and livestock-associated ST398 methicillin resistant Staphylococcus aureus strains.

Hallin M M   De Mendonça R R   Denis O O   Lefort A A   El Garch F F   Butaye P P   Hermans K K   Struelens M J MJ  

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20101209 2


BACKGROUND: Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) has documented the diversity of the genetic background of strains associated with healthcare (HA-MRSA), community (CA-MRSA) and livestock (LA-MRSA). The accessory and core-variable genomes of those strains however remain largely unknown. OBJECTIVE: To compare the genetic background and accessory and core variable gene content of ST398 LA-MRSA strains with those of HA-and CA-MRSA strains from the same region. METHO  ...[more]

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