Dataset Information


GC-rich Sequence Elements Recruit Polycomb Repressive Complex 2 (PRC2) in ES cells

ABSTRACT: Polycomb proteins are epigenetic regulators that localize to developmental loci in the early embryo where they mediate lineage-specific gene repression. In Drosophila, these repressors are recruited to sequence elements by DNA binding proteins associated with Polycomb repressive complex 2 (PRC2). However, the sequences that recruit PRC2 in mammalian cells have remained obscure. To address this, we integrated a series of engineered bacterial artificial chromosomes into embryonic stem (ES) cells and examined their chromatin. We found that a 44 kb region corresponding to the Zfpm2 locus initiates de novo recruitment of PRC2. We then pinpointed a CpG island within this locus as both necessary and sufficient for PRC2 recruitment. Based on this causal demonstration and prior genomic analyses, we hypothesized that large GC-rich elements depleted of activating transcription factor motifs mediate PRC2 recruitment in mammals. We validated this model in two ways. First, we showed that a constitutively active CpG island is able to recruit PRC2 after excision of a cluster of activating motifs. Second, we showed that two 1 kb sequence intervals from the E. coli genome with GC-contents comparable to a mammalian CpG island are both capable of recruiting PRC2 when integrated into the ES cell genome. Our findings demonstrate a causal role for GC-rich sequences in PRC2 recruitment and implicate a specific subset of CpG islands depleted of activating motifs as instrumental for the initial localization of this key regulator in mammalian genomes. Overall design: Analysis of YY1 binding in two cell types

INSTRUMENT(S): Illumina Genome Analyzer II (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Bradley E. Bernstein  

PROVIDER: GSE25197 | GEO | 2010-11-23



Similar Datasets

2010-11-23 | E-GEOD-25197 | ArrayExpress
2018-05-07 | PXD005821 | Pride
| GSE97805 | GEO
2016-07-26 | E-GEOD-81795 | ArrayExpress
2010-12-30 | GSE21482 | GEO
| GSE43915 | GEO
2015-01-27 | E-GEOD-43915 | ArrayExpress
2010-12-30 | E-GEOD-21482 | ArrayExpress
2012-06-11 | E-GEOD-38577 | ArrayExpress
2008-11-01 | GSE13084 | GEO