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Identification of differentially regulated genes in hematopoietic stem cells and URE leukemia cell line

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

OTHER RELATED OMICS DATASETS IN: PRJNA137817PRJNA141719PRJNA141721

ORGANISM(S): Mus musculus

PROVIDER: GSE27947 | GEO | 2012/09/14

SECONDARY ACCESSION(S): PRJNA137817

REPOSITORIES: GEO

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Identification of differentially regulated genes upon shRNA-mediated knock-down of HLX in the URE leukemia cell line

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Identification of differentially regulated genes upon shRNA-mediated knock-down of HLX in the URE leukemia cell line

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Project description:CD41 expression is associated with the earliest stages of mouse hematopoiesis. It is notably expressed on some cells of the intra-aortic hematopoietic clusters, an area where the first adult-repopulating hematopoietic stem cells (HSCs) are generated. Although it is generally accepted that CD41 expression marks the onset of primitive/definitive hematopoiesis, there are few published data concerning its expression on HSCs. It is as yet uncertain whether HSCs express CD41 throughout development, and if so, to what level. We performed a complete in vivo transplantation analysis with yolk sac, aorta, placenta, and fetal liver cells, sorted based on CD41 expression level. Our data show that the earliest emerging HSCs in the aorta express CD41 in a time-dependent manner. In contrast, placenta and liver HSCs are CD41⁻. Thus, differential and temporal expression of CD41 by HSCs in the distinct hematopoietic territories suggests a developmental/dynamic regulation of this marker throughout development.

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Project description:This SuperSeries is composed of the SubSeries listed below.

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