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Transcriptomic analysis of channel catfish intestine response to Aeromonas hydrophila infection correlated to skin integrity and fed state

ABSTRACT: The study focuses on the intestinal immune responses of channel catfish (Ictalurus punctatus) under various feeding and fin-clipping conditions after infection with Aeromonas hydrophila. Transcriptomic data revealed significant activation of pathogen recognition pathways (TLR signaling and C-type lectins) and pro-inflammatory cytokines, particularly in the fed groups. Histopathological findings at 2 hours post-challenge (HPC) showed minimal to moderate expansion of the lamina propria by edema in the intestines of the treated groups. At 4 HPC, mild to moderate edema with lymphocytes and plasma cells was observed, indicating early immune cell infiltration. By 8 HPC, degenerative changes in the superficial epithelium and hemorrhage were evident, especially in the fed fin-clipped group, which correlates with increased mortality in this group. The unfed groups demonstrated delayed but sustained immune responses, marked by lower histological damage compared to the fed groups. These findings provide insights into how nutritional status and fin-clipping shape the intestinal immune response and tissue damage during infection.

ORGANISM(S): Ictalurus punctatus

PROVIDER: GSE281206 | GEO | 2025/08/13

REPOSITORIES: GEO

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