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Genome-wide mapping of the Galleria mellonella transcription start sites during fungal infection and treatment

ABSTRACT: Galleria mellonella is an emerging invertebrate experimental model, but its current genome annotation lacks transcription start site (TSS) information. Because TSS annotation is essential for understanding transcriptional regulation and alternative promoter usage, we used LQ-ssCAGE to map CAGE-defined TSSs from 72 G. mellonella larval samples. Among these, 36 larvae were infected with the fungal pathogen Madurella mycetomatis and 36 served as healthy controls, allowing identification of TSSs under both infected and healthy conditions. Infected and healthy larvae were treated with itraconazole or ravuconazole, and total RNA was collected at 6, 30, 72, and 168 hours from biological triplicates for library preparation and sequencing. To assess key characteristics of the CAGE data, CAGE TSSs, tag clusters, TSS peaks, and putative enhancers were intersected with the existing genome annotation for annotation and characterization. This analysis identified two sets of TSS peaks, permissive and robust, representing the first draft TSS atlas for G. mellonella. The atlas-derived annotations showed a markedly stronger core promoter sequence signature than the NCBI reference annotation, supporting improved resolution and accuracy of TSS annotation in the atlas.

ORGANISM(S): Galleria mellonella

PROVIDER: GSE282923 | GEO | 2024/12/11

REPOSITORIES: GEO

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