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Uhrf1 determines osteophyte fate by regulating differentiation of synovial mesenchymal progenitor cells

ABSTRACT: This study investigates the role of Uhrf1 in osteophyte formation, building on previous findings of the role of Uhrf1 in cartilage proliferation and chondrogenic differentiation. We used mesenchymal stem cell-specific Uhrf1 knockout (cKO) mice to evaluate osteophyte development and cartilage degeneration at 2 and 12 weeks post-DMM surgery. At 2 weeks, cKO mice had significantly smaller osteophytes with reduced width, area, and maturity scores compared to controls. At 12 weeks, despite similar maturity scores, osteophytes in cKO mice were still smaller, and cartilage degeneration was more severe. Uhrf1 knockdown in human synovial cells led to decreased proliferation and chondrogenic differentiation. These results suggest that Uhrf1 is essential for early osteophyte formation and chondrogenesis, with its effects continuing as osteophytes mature. Additionally, osteophytes seem to protect against cartilage degeneration in OA, and RNA-seq analysis revealed that Uhrf1 deficiency upregulates genes that negatively affect cell devel

ORGANISM(S): Homo sapiens

PROVIDER: GSE296850 | GEO | 2026/05/12

REPOSITORIES: GEO

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Uhrf1 determines osteophyte fate by regulating differentiation of synovial mesenchymal progenitor cells [MBD-seq]

Project description:This study investigated the role of Uhrf1 in osteophyte formation, building on previous findings of its role in cartilage proliferation and chondrogenic differentiation. We used mesenchymal progenitor cell-specific Uhrf1 knockout (cKO) mice to evaluate osteophyte development and articular cartilage degeneration 2 and 12 weeks post-DMM (destabilization of the medial meniscus) surgery. Two weeks after DMM, cKO mice exhibited significantly smaller osteophytes with reduced width, area, and maturity scores compared to controls. Twelve weeks after DMM, despite similar maturity scores, osteophytes in cKO mice were still smaller, and articular cartilage degeneration was more severe in cKO. UHRF1 knockdown in human synovial cells led to decreased proliferation and chondrogenic differentiation with less methylated DNA. Integrated analyses with RNA-seq and MBD-seq revealed that UHRF1 deficiency upregulates genes that negatively affect cell development and growth including TORC1 and β-catenin-independent Wnt signaling. Among them, knockdown of NLK rescued chondrogenic differentiation, affected by siUHRF1. These results suggest that Uhrf1 in mesenchymal progenitor cells is essential for early osteophyte formation and chondrogenesis through DNA methylation, at least in part, followed by NLK gene suppression. Osteophytes play a protective role against articular cartilage degeneration in osteoarthritis.

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