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TBK1 enhances antifungal immunity by forming a SRC-TBK1 positive feedback loop in phagosomes of macrophages

ABSTRACT: TBK1 is a serine/threonine kinase known for its role in antiviral immunity. Here, we identify TBK1 as a key amplifier of antifungal immune responses. Using a mouse model of Candida albicans infection, we show that TBK1 is recruited to phagosomes via SRC-SHP2 signaling and phosphorylates SRC to establish a positive feedback loop. This enhances downstream cytokine production, including IL-6 and TNF-α, and promotes fungal clearance. Bone marrow-derived macrophages (BMDMs) from TBK1fl/fl and TBK1fl/fl Lyz2-Cre mice were stimulated with various fungal components, and RNA-seq was performed to assess transcriptional changes. Our findings define a new role for TBK1 in antifungal host defense and suggest TBK1 as a potential therapeutic target.

ORGANISM(S): Mus musculus

PROVIDER: GSE297902 | GEO | 2025/06/14

REPOSITORIES: GEO

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TBK1 enhances antifungal immunity by forming a SRC-TBK1 positive feedback loop in phagosomes of macrophages

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