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Methylome profiling of SetDB1 deficient ESCs reveals coordinated epigenetic cross-talk during pluripotency

ABSTRACT: DNA methylation profiling using whole-genome bisulfite sequencing of conditional SetDB1 knockout (KO) mouse embryonic stem cells (ESCs). Induction with 4-hydroxytamoxifen (4-OHT) facilitated the deletion of Setdb1 floxed exons 15 and 16, resulting in loss of SetDB1. Methylomes were profiled in wildtype (WT) serum- and 2i-cultured ESCs, and on multiple days after inducing SetDB1 depletion. Study also includes methylome profiles of ESCs undergoing simultaneous SetDB1 depletion and 2i-adaptation.

ORGANISM(S): Mus musculus

PROVIDER: GSE299458 | GEO | 2026/06/10

REPOSITORIES: GEO

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