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RNA Sequencing Reveals Differential Gene Expression in Human Brain Microvascular Endothelial Cells (HBMECs) Subjected to Oxygen-Glucose Deprivation/Reperfusion (OGD/R)

ABSTRACT: Human brain microvascular endothelial cells (HBMECs) are a critical component of the blood-brain barrier (BBB), and their stability is paramount for BBB integrity, particularly under pathological stress such as ischemic stroke. This study employed an *in vitro* model of ischemia-reperfusion injury using oxygen-glucose deprivation followed by reperfusion (OGD/R) on cultured HBMECs. To comprehensively characterize the transcriptional response, we performed high-throughput RNA sequencing (RNA-seq) on both OGD/R-treated and control (normoxic) HBMEC samples. Our analysis identified a significant number of differentially expressed genes (DEGs) associated with the OGD/R challenge. These DEGs encompass genes involved in key biological processes pertinent to endothelial cell dysfunction, BBB disruption, inflammatory responses, oxidative stress, apoptosis, and potential protective/repair mechanisms. This dataset provides a detailed transcriptional profile of HBMECs undergoing simulated ischemia-reperfusion injury. It serves as a valuable resource for elucidating the molecular mechanisms underlying BBB compromise in ischemic stroke and for identifying novel potential therapeutic targets aimed at preserving BBB function and mitigating neuronal damage.

ORGANISM(S): Homo sapiens

PROVIDER: GSE303528 | GEO | 2026/06/30

REPOSITORIES: GEO

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Project description:Cerebral ischemia/reperfusion injury (CI/RI), including neurological behavior deficits, cerebral infarction, blood-brain barrier (BBB) dysfunction, and neuroinflammation, et al. is a severe challenge in treatment of ischemic stroke. Traditional Chinese medicine (TCM) with the characteristics of multi-components and multi-functions for multiple targets/pathways has been historically used in the treatment of stroke and post stroke recovery in China. QiShenYiQi (QSYQ), a representative component-based Chinese medicine, is capable of reducing organ injury caused by ischemia/reperfusion via multiple mechanisms. Recently, we have previously reported that the positive action of QSYQ against the acute phase of CI/RI is partly via modulation of neuroinflammatory response. However, the effects and underlying mechanisms of QSYQ on subacute phase of CI/RI remain unknown, which are aimed to investigate in present study. The pharmacological action of QSYQ treatment on brain damage was estimated in the experimental stroke rats underwent 90 min ischemia and 8 days reperfusion by assessing the neurological and locomotor deficits, cerebral infarction, brain edema, and BBB integrity. Furthermore, we used TMT-based quantitative proteomics technology to identify significantly differentially expressed proteins following QSYQ treatment, which could give important clues for revealing the possible mechanism underlying the positive role of QSYQ in CI/RI. Besides, immunohistochemistry, western blot analysis, RT-qPCR, and rat ELISA kits were executed to further verify the accuracy of proteomics analysis results. As a consequence, we found that treatment with QSYQ (600mg/kg) for 7 days obviously improved neurological and behavioral impairment, attenuated infarct volume and brain edema, and alleviated BBB breakdown in stroke rats. Bioinformatics analysis suggested that differentially expressed protein galectin-3 mediated inflammatory response was closely related to the beneficial effect of QSYQ. Results of immunohistochemistry, western blot analysis and RT-qPCR showed that the model rats treated with QSYQ (600mg/kg) markedly downregulated the mRNA and protein expression level of galectin-3 in brain tissues compared to the untreated stroke rats. In addition, the decrease of mRNA level in brain tissues and contents in serum of TNF-α and IL-6 following QSYQ treatment were also observed. These interesting finding manifested that the effective action of QSYQ against subacute phase of CI/RI was partly via regulating galectin-3 mediated inflammatory reaction.

Dataset Information

RNA Sequencing Reveals Differential Gene Expression in Human Brain Microvascular Endothelial Cells (HBMECs) Subjected to Oxygen-Glucose Deprivation/Reperfusion (OGD/R)

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