Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Epithelial chemokine CCL25 integrates T cell development and intestinal homeostasis

ABSTRACT: The chemokine receptor CCR9 regulates thymic colonization of T-progenitors and intestinal accumulation of TCRgd+ intraepithelial cells (IELs). CCR9-ligand chemokine CCL25 is expressed predominantly in the thymus and the small intestine. By engineering tissue-specific CCL25-deficient mice, we demonstrate that CCL25 locally produced by thymic and intestinal epithelial cells individually supports developing T cell localization in the thymus and TCRgd+ IEL accumulation in the small intestine, respectively. More interestingly, our results reveal that thymic epithelial CCL25 facilitates T-cell positive selection in the thymic cortex. We further find that intestinal epithelial CCL25 promotes nutrient sensing in the small intestine. These results indicate that epithelial CCL25 directs thymic T-cell development and intestinal homeostasis in a tissue-specific paracrine manner.

ORGANISM(S): Mus musculus

PROVIDER: GSE310955 | GEO | 2026/04/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

MAIT cells at different developmental stages egress the thymus to colonize specific tissues and sense increases in peripheral ligands

Project description:MAIT but not iNKT cells can be selected by thymic epithelial cells, while both require CD4+CD8+ thymocytes for maturation. MAIT and iNKT cells have similar developmental dynamics and thymic-residency properties but proliferate at different developmental stages.MAIT cells exit the thymus in an S1PR-dependent manner at different maturation stages to colonize specific peripheral organs.PLZF+Tbet-RORgt- MAIT cells preferentially colonize the intestine in a CCR9-dependent manner.Thymic output contributes to peripheral MAIT and iNKT cell numbers at steady state. Thymic MAIT17 cells are activated and expand in colitis and after bacterial exposure.

2026-04-13 | GSE308509 | GEO

Epithelial chemokine CCL25 integrates T cell development and intestinal homeostasis

Project description:Epithelial chemokine CCL25 integrates T cell development and intestinal homeostasis

| PRJNA1367597 | ENA

Intestinal epithelial and intraepithelial T cell crosstalk mediates a dynamic response to infection

Project description:Oral infection of mice with Salmonella Typhimurium leads to rapid changes in small intestinal TCRgd IEL behavior. How these changes are induced is currently unknown. By analyzing the transcriptome of TCRgd and intestinal epithelial cells in the steady state and after infection, we find a potential role for epithelial cell MYD88 expression in regulating the IEL response. By additionally analyzing the transcriptome of epithelial cell-specific, conditional Myd88 knockout animals after Salmonella infection, we address the role of epithelial cell Myd88 signaling in regulating the IEL and EC response to Salmonella infection.

2017-09-21 | GSE97184 | GEO

High-throughput RT-PCR analysis of Colon Adenocarcinoma Stem Cell Lines

Project description:Human early stage colon adenocarcinoma stem cell line was FACS-sorted for CCR9- and CCR9+ cell groups and differential expression was identified via Wafergen SmartChip RT-PCR gene expression profiling. CCR9- and CCR+ cells were FACS-sorted. 24 hours afterwards, cells were treated with 100ng/ml human CCL25 for 30 min. RNA was extracted from both populations using PureLink RNA Mini kit (Invitrogen) and analyzed using the SmartChip Real-Time PCR System

2012-07-01 | E-GEOD-32290 | biostudies-arrayexpress

Construction of small intestine-like tissue by reproducing interstitial flow on-chip

Project description:Significant progress has been recently achieved in generating in vitro human small intestine models. Nonetheless, there remains ample opportunity for enhancement, both in terms of structure and function, within the current model. In this study, our objective is to construct a multilayered small intestinal tissue composed of an intestinal epithelium, supportive mesenchymal cells, and an extracellular matrix. We developed a multilayered small intestinal tissue by differentiating human induced pluripotent stem cells on a microfluidic device capable of replicating interstitial flow. Under the interstitial flow exposure, a three-dimensional villus-like epithelium and mature intestinal epithelial cells, including polarized enterocytes or goblet cells, were observed in the small intestine tissue-on-a-chips. Further analysis revealed that intestinal fibroblasts and collagen fiber are localized to the basolateral side of the intestinal epithelium. Moreover, we confirmed that small intestine tissue-on-a-chips are useful for pharmaceutical and infectious disease research. Our small intestine tissue-on-a-chips not only overcome the limitations of conventional small intestine models but also offer a unique opportunity to understand the mechanisms underlying intestinal tissue development.

2024-11-16 | PXD047318 | JPOST Repository

Thymic CD11c+ cells [scRNA-seq]

Project description:This study investigates the dynamics of thymic dendritic cells (DCs) under tumor-bearing conditions and the role of CCR9 in regulating thymic DC homeostasis. CD11c⁺ thymic cells were enriched from wild-type (WT) and CCR9 knockout (CCR9 KO) mice subjected to different experimental treatments. Four experimental groups were analyzed: CCR9 WT mice treated with PBS for 2 weeks CCR9 WT mice bearing MC38 tumors for 2 weeks CCR9 WT mice bearing MC38 tumors for 3 weeks CCR9 KO mice bearing MC38 tumors for 3 weeks

2026-02-03 | GSE317744 | GEO

Intestinal and thymic tuft cell RNA-seq

Project description:The goal of the study was to sequence mRNA from tuft cells (identified as CD45-;EpCAM+;IL-25+ using Flare25 reporter mice) in the epithelia of thymus and small intestine. As a control, non-tuft epithelial cells (CD45-;EpCAM+;IL-25-) were also isolated. The data were used to compare tuft cell markers between the small intestine and thymus.

2018-05-19 | GSE114668 | GEO

Methylation array data of thymic epithelial tumors (TC, NET, THYM)

Project description:Methylation microarray data (Illumina 850K) of 52 thymic epithelial tumors. 13 patients with thymoma A and B, 32 thymic carcinoma (TC) and 7 neuroendocrine tumors of the thymus (NET).

| EGAD00010002355 | EGA

Genome-wide maps of Cbfb variants bound regions in E12.5 lineage negative fetal liver cells and adult thymocytes.

Project description:Multi-potent hematopietic progenitors must acquire thymus-homing capacity to initiate T lymphocyte development. Despite its importance, the transcriptional program underlying this process remains elusive. Cbfβ forms transcription factor complexes with Runx proteins, and here we sho that Cbfβ2, encoded by an RNA splice variant of the Cbfb gene, is essential for differentiation of IL7R+PIRhi thymic-homing progenitors in the mouse fetal liver. Cbfβ2 binds to cell-type specific enhancers and induces expression of the principal thymus-homing receptor CCR9. Like in mouse, an alternative splicing event generates Cbfβ2-specific mRNA whose products controls CCR9 expression and thymus homing in zebrafish. Our findings indicate that functional diversification of Runx transcription factor complexes via alternative splicing emerged early in evolution to support a key step in T cell development.

2017-11-09 | GSE90794 | GEO

RNA-sequencing of coding RNA from human purified intestinal epithelial samples and organoids derived from foetal and paediatric small and large intestine

Project description:Human intestinal epithelial organoids (IEO) culture models are rapidly emerging as novel experimental tools to investigate fundamental aspects of intestinal epithelial (patho)physiology. Cellular source and culture protocols vary between different IEO models and reliable markers for their characterization/validation are currently limited. Here, we provide the following reference datasets of transcriptomic profiling by RNA-sequencing: Purified intestinal epithelial cells (EpCAM+) from paediatric ileum and colon, Intestinal organoid cultures from paediatric ileum and colon, Purified intestinal epithelial cells (EpCAM+) from foetal small intestine and foetal large intestine, Intestinal organoid cultures from foetal small intestine and foetal large intestine, Intestinal organoid cultures derived from induced pluripotent stem cells.<br> Complementary data from methylation profiling on the same samples have been deposited at ArrayExpress under accession number E-MTAB-4957 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4957 ).</br>

2018-01-20 | E-MTAB-5015 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data