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Transcriptomics Identifies Mild Hyperthermia-Conditioned Tumor Supernatant as an Inducer of HepG2 Maturation

ABSTRACT: This study aimed to explore whether tumor supernatant (TSN) under mild hyperthermia induces the transformation of HepG2 cells into mature hepatocyte-like cells and to identify the underlying mechanisms involved in this process. HepG2 cells were treated at a mild hyperthermic temperature of 42.5°C for 60 minutes, and the resulting supernatant was collected and labeled as 42.5TSN. As a control, HepG2 cells were also treated at a normal temperature of 37°C for 60 minutes, and the supernatant was collected and labeled as 37TSN. The collected TSN was then concentrated and applied to untreated normal HepG2 cells to detect differential gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE313027 | GEO | 2026/07/01

REPOSITORIES: GEO

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