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SnRNA-Seq from abdominal and gluteofemoral human adipose tissue

ABSTRACT: Adipose tissue biopsies were collected from subcutaneous abdominal and gluteofemadipose tissue from 5 healthy and 5 PCOS women. After isolation, nuclei were counted with a Countess II automated cell counter (Thermofisher Scientific). Single nuclei suspension (56K/mL) was distributed into eight wells of a 384-well source plate (Takara Bio USA, San Jose, CA) and dispensed onto a iCELL8 350v Chip (Takara Bio USA) using an iCELL8 MultiSample NanoDispenser (Takara Bio USA). We distributed the ABD and GF-SVF of each subject on the same chip. In brief, mRNA from single cells were isolated, converted to full-length cDNA prior to unbiased amplification of 3’ and 5’ ends using SMART-Seq® ICELL8® Application Kit (Takara Bio, USA). Multiplexed sequencing libraries were generated from cDNA using the Illumina Nextera XT protocol and 150bp paired-end sequencing was performed on an Novogene NovaSeq X Plus instrument.

ORGANISM(S): Homo sapiens

PROVIDER: GSE322696 | GEO | 2026/06/01

REPOSITORIES: GEO

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