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RNA binding protein YWHAZ mediates translation of specific mRNA and regulates cell Proliferation and apoptosis in diabetic foot ulcer

ABSTRACT: YWHAZ was a significantly up-regulated and validated RBP in DFU by RT-qPCR and immunohistochemistry. Cellular experiments revealed that Si_YWHAZ facilitated proliferation and migration while inhibiting apoptosis, consistent with its upregulation in DFU. RNA-seq identified 1072 DEGs in Si_YWHAZ cells. Up-regulated genes were significantly enriched in cell proliferation-related processes and included AREG, FOSL1, HAS2 and IL7R, whereas down-regulated genes were associated with cell adhesion, such as LAMB3, SLAMF7, COL12A1 and ITGA5. IRIP-seq results demonstrated that YWHAZ interacts with plentiful of mRNAs and genomic location toward the CD and introns region. As well, ABLIFE algorithm indicated that YWHAZ bound to GC-rich motifs. Integrated iRIP-seq and RNA-seq analyses identified 57 DEGs that were selectively bound by YWHAZ, most of which were down-regulated. Notably, SREBF1, which was positively associated with type 2 diabetes risk. KEGG pathway analysis revealed SREBF1 is enriched in insulin resistance and insulin signaling pathway

ORGANISM(S): Homo sapiens

PROVIDER: GSE327462 | GEO | 2026/07/29

REPOSITORIES: GEO

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YWHAZ is an Invasion and Metastasis promoting genes of Lung cancer

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Transcriptomic analysis of Ywhaz knockout in preimplantation mouse embryos

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