Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling of the Epidermis of Naked Mole-RatsTreated with DMBA and TPA

ABSTRACT: We compared expressions of cancer-related genes (tumor suppressor genes and oncogenes) in the skin epidermis between Naked mole-rats treated with DMBA/TPA and control animals. Bulk RNA-seq analyses revealed that in contrast to control animals, epidermis of Naked mole-rats treated with DMBA/TPA is characterized by increased expression of anti-cancer genes, activation of genes controlling ferroptosis, as well as by downregulation of numerous oncogenes including components of NF-kB, Wnt and tyrosine kinase signaling pathways.

ORGANISM(S): Heterocephalus glaber

PROVIDER: GSE334586 | GEO | 2026/07/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional profiling of Mouse Epidermis Treated with DMBA and TPA

Project description:We compared expressions of cancer-related genes (tumor suppressor genes and oncogenes) in the skin epidermis between FVB mice treated with DMBA/TPA and control animals. Bulk RNA-seq analyses revealed that in contrast to control animals, epidermis of mice treated with DMBA is characterized by upregulation of the genes enriched for immune response pathways, whereas downregulated genes were involved in the control of extracellular matrix remodeling and wound healing. In mouse epidermis treated by DMBA/TPA, transcriptional response to DMBA/TPA at the pre-tumor stage was accompanied by upregulation of the genes controlling epidermal differentiation, oncogenes including the components of Wnt signaling pathway, as well as of selected tumor suppressor genes.

2026-07-09 | GSE334587 | GEO

single-cell RNA-seq of mouse back skin treated with TPA

Project description:Mice are cancer-prone, whereas naked mole-rats are cancer-resistant. To test the discrepancies between how mouse and naked mole-rat skin responds to carcinogens, we topically treated animals with DMBA followed by TPA. In this dataset, we additionally perform single-cell RNA-seq on back skin in mice treated exclusively with TPA as a control.

2025-11-19 | E-MTAB-13556 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (Essen) 2022

Project description:We generated single cell transcriptomes from full thickness skin biopsies in naked mole-rat to quantify the skin cell types found in this species (control samples). To study if and how naked mole-rat skin changes upon exposure to a carcinogen, we performed a classical two-stage skin carcinogenesis experiment traditionally performed in mice, wherein cancer is initiated by a single application of 7,12-dimethylbenz[a]-anthracene (DMBA) followed by repeated treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) to drive cell proliferation. After 12 weeks, full thickness skin biopsies were collected and used to generate single cell transcriptomes (treatment samples).

2023-11-21 | E-MTAB-12459 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK B) 2022

2023-11-21 | E-MTAB-12461 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK A) 2022

2023-11-21 | E-MTAB-12451 | biostudies-arrayexpress

Transcriptional profiling of the Epidermis of Naked Mole-RatsTreated with DMBA and TPA

Project description:Transcriptional profiling of the Epidermis of Naked Mole-RatsTreated with DMBA and TPA

| PRJNA1475988 | ENA

African spiny mice show resistance to DMBA/TPA-induced squamous carcinogenesis with distinct benign tumor profile [acomys_dt]

Project description:African spiny mice, Acomys cahirinus (Acomys), is an emerging mammalian model for regeneration studies because of its ability to morphologically and functionally regenerate several tissues. However, as limited regenerative abilities in most mammals are viewed as an antitumor strategy, it is intriguing how Acomys balance regeneration and tumor suppression. To date, no spontaneous tumors have been reported in Acomys, suggesting it may be resistant to cancer. In this study, we use in vitro and in vivo approaches to investigate if A. cahirinus is susceptible to carcinogenesis. We report that, like mice, two oncogenic hits - activation of oncogenic RasG12V and inactivation of p53 or pRb - are sufficient to transform skin fibroblasts from A. cahirinus. However, surprisingly, active p53 dramatically reduces the tumor forming ability of the transformed fibroblasts. In the DMBA/TPA treatment model of in vivo skin carcinogenesis with animals aged 1-1.5 years, C57BL/6 laboratory mice formed pre-malignant skin tumors in 22 weeks, while A. cahirinus did not show any effects. A longer TPA treatment period of 30 weeks induced benign sebaceous adenomas in A. cahirinus. This showed that A. cahirinus was resistant to squamous skin carcinogenesis typically induced by DMBA/TPA treatment. A. cahirinus showed significantly higher upregulation of immune response and differentially expressed tumor suppressors and oncogenes in DMBA/TPA-treated skin when compared to mice. Wnt/β-catenin signaling, a major mediator of squamous carcinogenesis associated with DMBA/TPA treatment, was inhibited in A. cahirinus. Several Wnt/β-catenin targets and effector transcription factors, especially Lef1, were transcriptionally downregulated in A. cahirinus. Overall, our study shows that A. cahirinus may be resistant to DMBA/TPA-induced squamous carcinogenesis, and this resistance may be mediated by a strong immune response and the inhibition of Wnt/β-catenin signaling.

2025-10-15 | GSE287009 | GEO

African spiny mice show resistance to DMBA/TPA-induced squamous carcinogenesis with distinct benign tumor profile [acomys_irrad]

2025-10-15 | GSE287008 | GEO

Expression data from sorted epithelial CD34+ expressing cells from DMBA/TPA induced skin tumors

Project description:Transcriptional profile of control and VEGF overexpressing FACS-isolated CD34+ Cancer stem cells from DMBA/TPA induced skin tumours

2011-09-07 | GSE31465 | GEO

Transcriptional profiling of epidermal keratinocytes from Naked mole-rats and mice

Project description:We compared expressions of cancer-related genes (tumor suppressor genes and oncogenes) between homeostatic epidermal keratinocytes from Naked mole-rats and FVB mice. Bulk RNA-seq analyses revealed that homeostatic epidermal keratinocytes in Naked mole-rats maintain high expression of tumor suppressor genes that inhibit cell proliferation and activity of pro-oncogenic Wnt, IGF and Hedgehog signaling pathways, stimulate apoptosis and DNA repair, which is associated with downregulation of several key oncogenes stimulating skin carcinogenesis. In contrast to Naked mole-rats, mouse keratinocytes predominantly express tumor suppressor genes linked to cell adhesion, growth factors signaling, nuclear receptors and transcriptional regulators, as well as show decreased expression of oncogenes driving carcinogenesis outside of the skin.

2026-07-09 | GSE334395 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data