Transcriptomics

Dataset Information

0

Effects of 3D and 2D culture on gene expression of HCT116 cells


ABSTRACT: Accurate preclinical models that recapitulate in vivo tumor pathophysiology are essential for reliable assessment of anticancer drug efficacy. Three-dimensional (3D) cell culture systems, which better mimic the in vivo microenvironment, have garnered significant attention. However, high throughput production of uniformly sized spheroids remains a key challenge for drug screening. we developed a PDMS substrate with hexagonally arrayed U-shaped microwells (HAUM PDMS) for high-throughput culture of uniformly sized spheroids. Transcriptome sequencing was performed on HCT116 spheroids harvested from 500 µm HAUM PDMS substrates and on 2D-cultured cells after 3 days. The results showed that transcriptome analysis indicated that up-regulated genes in spheroids were mainly related to cell division and proliferation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE344100 | GEO | 2026/08/18

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2026-06-11 | GSE335056 | GEO
2023-06-13 | GSE234529 | GEO
2024-01-03 | GSE236576 | GEO
2025-02-15 | PXD060857 |
2026-09-07 | PXD065781 | Pride
2012-06-11 | E-GEOD-36480 | biostudies-arrayexpress
2026-08-03 | GSE304137 | GEO
2026-03-31 | GSE322504 | GEO
2022-01-20 | GSE190296 | GEO
2022-11-07 | GSE199753 | GEO