Effects of 3D and 2D culture on gene expression of HCT116 cells
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ABSTRACT: Accurate preclinical models that recapitulate in vivo tumor pathophysiology are essential for reliable assessment of anticancer drug efficacy. Three-dimensional (3D) cell culture systems, which better mimic the in vivo microenvironment, have garnered significant attention. However, high throughput production of uniformly sized spheroids remains a key challenge for drug screening. we developed a PDMS substrate with hexagonally arrayed U-shaped microwells (HAUM PDMS) for high-throughput culture of uniformly sized spheroids. Transcriptome sequencing was performed on HCT116 spheroids harvested from 500 µm HAUM PDMS substrates and on 2D-cultured cells after 3 days. The results showed that transcriptome analysis indicated that up-regulated genes in spheroids were mainly related to cell division and proliferation.
ORGANISM(S): Homo sapiens
PROVIDER: GSE344100 | GEO | 2026/08/18
REPOSITORIES: GEO
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