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The Effects of Nanog HD Mutants on Mouse Embryonic Stem Cells

ABSTRACT: We examined global gene expression patterns of mouse embryonic stem cells overexpressing EGFP, Nanog wild-type, or Nanog L122A mutants in normal, "-LIF" or "+RA" culture conditions by RNA-seq experiments. In “+RA” culture conditions, the expression patterns of the RA-responsive genes were slightly different in WT transfectants and more different in L122A transfectants compared with EGFP transfectants. These results suggested that L122A transfectants showed partial resistant activity against RA-induced differentiation, which was not found in WT mNANOG transfectants.

ORGANISM(S): Mus musculus

PROVIDER: GSE66127 | GEO | 2015/02/21

SECONDARY ACCESSION(S): PRJNA275930

REPOSITORIES: GEO

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The Effects of Nanog HD Mutants on Mouse EMbryonic Stem Cells

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