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Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria

ABSTRACT: A prototype oligonucleotide microarray was designed to detect and identify viable bacterial species with the potential to grow of common beer spoilage microorganisms from the genera Lactobacillus, Megasphaera, Pediococcus and Pectinatus. Probes targeted the intergenic spacer regions (ISR) between 16S and 23S rRNA, which were amplified in a combination of reverse transcriptase (RT) and polymerase chain reaction (PCR) prior to hybridization. This method allows the detection and discrimination of single bacterial species in a complex sample. Furthermore, microarrays using oligonucleotide probes targeting the ISR allow the distinction between viable bacteria with the potential to grow and non-growing bacteria. The results demonstrate the feasibility of oligonucleotide microarrays as a contamination control in food industry for the detection and identification of spoilage microorganisms within mixed population. Keywords: microarray, oligonucleotide, species-specific, detection, beer spoilage bacteria

OTHER RELATED OMICS DATASETS IN: PRJNA99965

ORGANISM(S): Lacticaseibacillus casei Pectinatus cerevisiiphilus Pectinatus frisingensis Schleiferilactobacillus perolens Pediococcus damnosus Levilactobacillus brevis Loigolactobacillus coryniformis Megasphaera cerevisiae Pediococcus inopinatus

PROVIDER: GSE7840 | GEO | 2007/05/23

SECONDARY ACCESSION(S): PRJNA99965

REPOSITORIES: GEO

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Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria

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