Other,Multiomics

Dataset Information

1K

Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria


ABSTRACT: A prototype oligonucleotide microarray was designed to detect and identify viable bacterial species with the potential to grow of common beer spoilage microorganisms from the genera Lactobacillus, Megasphaera, Pediococcus and Pectinatus. Probes targeted the intergenic spacer regions (ISR) between 16S and 23S rRNA, which were amplified in a combination of reverse transcriptase (RT) and polymerase chain reaction (PCR) prior to hybridization. This method allows the detection and discrimination of single bacterial species in a complex sample. Furthermore, microarrays using oligonucleotide probes targeting the ISR allow the distinction between viable bacteria with the potential to grow and non-growing bacteria. The results demonstrate the feasibility of oligonucleotide microarrays as a contamination control in food industry for the detection and identification of spoilage microorganisms within mixed population. Keywords: microarray, oligonucleotide, species-specific, detection, beer spoilage bacteria Overall design: Each oligonucleotide species-specific probe was spotted in 70 replicate spots on the microarray. Nine single beer spoilage bacteria species were detected with the developed prototype oligonucleotide microarray and also three mixes of two different beer spoilage bacteria species.

OTHER RELATED OMICS DATASETS IN: PRJNA99965

INSTRUMENT(S): Technical Microbiology-Viable beer spoilage bacteria microarray V1

SUBMITTER: Daniel Gilbert Weber 

PROVIDER: GSE7840 | GEO | 2007-05-23

SECONDARY ACCESSION(S): PRJNA99965

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE7840_RAW.tar Raw
filelist.txt Txt
Items per page:
1 - 2 of 2
altmetric image

Publications

Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria.

Weber D G DG   Sahm K K   Polen T T   Wendisch V F VF   Antranikian G G  

Journal of applied microbiology 20080910 4


AIMS: The design and evaluation of an oligonucleotide microarray in order to detect and identify viable bacterial species that play a significant role in beer spoilage. These belong to the species of the genera Lactobacillus, Megasphaera, Pediococcus and Pectinatus. METHODS AND RESULTS: Oligonucleotide probes specific to beer spoilage bacteria were designed. In order to detect viable bacteria, the probes were designed to target the intergenic spacer regions (ISR) between 16S and 23S rRNA. Prior  ...[more]

Similar Datasets

| PRJNA99965 | ENA
| S-EPMC8391359 | BioStudies
| S-EPMC3029383 | BioStudies
| S-EPMC7380088 | BioStudies
| S-EPMC3765258 | BioStudies
| S-EPMC6974642 | BioStudies
| S-EPMC2746227 | BioStudies
| S-EPMC4309717 | BioStudies
| S-EPMC6293109 | BioStudies
1995-01-01 | S-EPMC167419 | BioStudies